Figure 7.

Time course of activation of KCC at 37°C after NEM pretreatment at 0°C. Rabbit red cells (not density separated) were treated twice with 2 mM NEM at 0°C as in Fig. 6, then washed in cold medium. One aliquot of cells was suspended in isosmotic flux medium and incubated at 37°C for 20 min before adding ⁸⁶Rb⁺ at t = 0 (•). The remaining cells were kept on ice for 20 min, and then suspended in 37°C flux medium containing ⁸⁶Rb⁺ at t = 0 (▴; mean ± range of two suspensions). The curve through the points represents with a lag time of 8.9 min. Control cells were not exposed to NEM (▪).