Figure 8.

Time course of activation of KCC at 25°C after NEM pretreatment at 0°C. Rabbit red cells (not density separated) were pretreated twice with NEM as in Fig. 6 and Fig. 7. Cells were then suspended at 2% hematocrit in flux medium at either 37°C (•) or on ice (▴; mean ± range of two suspensions). After 20 min, the suspensions were moved to a 25°C bath, and, after allowing 3 min for the temperature to equilibrate, ⁸⁶Rb⁺ was added at t = 0. Control cells were not exposed to NEM (▪). The curve through the ▴ data represents with a lag time of 79 min.