Figure 1. Hypoxia induces D3 activity and mRNA.

(A) Endogenous D3 activity in SK-N-AS neurons, rat neonatal cardiomyocytes, NCLP6E hepatocytes, choriocarcinoma cells (JEG-3 cells), endometrial cells (ECC-1 cells), and AG04526 fibroblasts exposed to normoxia (21% O₂) versus hypoxia (1% O₂) for 24 h. Values are mean ± SEM of 2 or 3 cell plates; mean of 3 experiments is shown for each cell type. *P < 0.005. (B) Northern blot analysis of total RNA obtained from SK-N-AS or NCLP6E cells exposed to hypoxia versus normoxia for 24 h. Lanes were run on the same gel but were noncontiguous. (C) D3 activity and Northern blotting in NCLP6E cells exposed to continuous normoxia (condition A), continuous hypoxia (condition B), or transient hypoxia for 24 h followed by normoxia (condition C). Representative experiment with mean ± SEM of 2 cell plates is shown; this experiment was reproduced.