Figure 3. Renin expression in TgM.

(A–D) Kidneys were isolated from 8-week-old Ren-1^C BAC TgM (4 males [M] and 4 females [F] for each genotype [WT and mut] in lines 546, 525, 399, and 1224), and the RNA was analyzed. (A and C) Each value represents the ratio of Tg Ren-1^C (Tg) expression to that of endogenous Ren-2 and Ren-1^D (endo.), which served as an internal control. Relative Tg/endo. values for each individual are shown after normalization to the average value of the WT group, which was arbitrarily set at 100. Each sample was analyzed at least 3 times, and the means ± SD are shown only for lines 546 and 399. ND, not detected. (B and D) The means ± SD of each group in A and C are summarized. Results are shown also for lines 525 and 1224. P values were determined by 2-tailed Student’s t test. (E–G) RNA was isolated from various tissues of non-Tg (E), CNRE Tg (F), and RP-2 Tg (G) animals (8-week-old). Endogenous Ren-1^C, Tg Ren-1^C (Tg), and Gapdh gene expression was analyzed. Lines in F indicate that lanes were run on the same gel but were noncontiguous. (H) The SMG was isolated from Ren-1^C TgM (8-week-old), and Tg Ren-1^C (Tg), endogenous Ren-2 and Ren-1^D (endo.), and Gapdh gene expression was analyzed. Below, the relative renin/GAPDH value for each individual mouse was calculated for both Tg and endogenous (endo.) renin genes and normalized to the average value of the WT male group, arbitrarily set at 100. Values are mean ± SD.