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. 2007 Aug 23;26(18):4053–4065. doi: 10.1038/sj.emboj.7601834

Figure 6.

Figure 6

The exo70-45 sec3ΔN double mutant is defective in secretion. (A) The exo70-45 sec3ΔN double mutant blocks the secretion of Bgl2. For exo70-45 and exo70-45 sec3ΔN, cells were cultured at 34°C, and then shifted to 18°C for 6 h. For sec6-4, cells were cultured at 25°C and shifted to 37°C for 1 h. The internal and external pools of Bgl2 were separated and subjected to Western blot with anti-Bgl2 antibody. (B) Thin-section electron microscopic analysis of exo70-45, sec3ΔN and exo70-45 sec3ΔN cells. Cells were grown to early log phase at 34°C, and then shifted to 18°C for 6 h before being processed for EM. Bars, 1 μm.