Table 1.
Exo70 Domain D mutants
| Mutants | Mutation sites | Growth of exo70 | Growth of exo70 sec3ΔN |
|---|---|---|---|
| Class I mutants | |||
| exo70-31 | K562, K564, K567 | Normal | Normal |
| exo70-33 | K574, K578 | Normal | Normal |
| exo70-43 | K537, K538, K540, K542 | Normal | Normal |
| Class II mutants | |||
| exo70-29 | K548, R550, K551 | Normal | Defective (⩽25°C) |
| exo70-47 | R595 | Normal | Severely defective |
| exo70-45 | K601, R604, K605, H606, K608 | Normal | Severely defective |
| exo70-41 | R595, K597 | Normal | Near lethal |
| Class III mutants | |||
| exo70-2945 | K548, R550, K551, K601, R604, K605, H606, K608 | Mildly defective (⩽25°C) | Lethal |
| exo70-35 | R591, R595, K597 | Defective (⩽25°C) | Lethal |
| exo70-2941 |
K548, R550, K551, R595, K597 |
Defective (⩽25°C) |
Lethal |
| *The growth defects of exo70-29 sec3ΔN, exo70-2945, exo70-2941 and exo70-35 strains are more readily observed at low temperatures (⩽25°C). | |||
| **Please note that exo70-41 and exo70-35 differ by one amino acid. | |||