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. 2007 Aug 30;26(18):4149–4159. doi: 10.1038/sj.emboj.7601840

Figure 2.

Figure 2

D-Ser accumulation on the surfaces of damaged motoneurons at earlier stages and D-Ser distribution in activated microglia and astrocytes at an end stage in spinal cords from ALS mice. (A) Double IF analysis showed that D-Ser accumulated on the NeuN- or SMI32-positive vacuolated motoneurons at the lumbar spinal cords from ALS mice at an age of 9 weeks. (B, C) D-Ser was colocalized with both IB4- and GFAP-positive cells, in lumbar spinal cords of ALS mice at 21 weeks. Areas shown are magnified spinal ventral horns (A, B), and spinal white matter (C). For the double immunostaining, the rabbit polyclonal anti-D-Ser antibody was used together with mouse monoclonal anti-NeuN antibody (A), SMI32 (A), IB4 (B, C), or mouse monoclonal anti-GFAP antibody (B, C). Arrows indicated merged neurons and arrowheads showed merged glia. Scale bars=50 μm.