Figure 2.

Effects of EUK-134 treatment on KA-induced changes in NF-κB- and AP-1-binding activity. Control and KA-treated rats were killed at 16 h or 5 days after KA injection. Nuclear extracts from control, KA, and KA & EUK groups were prepared from piriform cortex (A and C) and hippocampus (B and D). Aliquots (5-μg proteins) were incubated with labeled NF-κB (A and B) or AP-1 (C and D) probes, and bound nucleotides were separated from free probes by EMSA. Gels were dried and analyzed with a PhosphorImager (Molecular Dynamics). For each group, samples from three representative animals are shown. Lane F contained no nuclear extracts as a negative control. Arrows on the right correspond to NF-κB- or AP-1-specific binding complexes.