Figure 10. IC neurones can be electrically coupled.

A, a grabbed video image of a clump of ∼6 granule cells in which 2 cells were subject to simultaneous voltage clamp by means of separate patch-clamp amplifiers. B, the upper panel is a record of the voltage commands to the patch electrodes: right electrode in A, steady holding potential, -60 mV; left electrode, -80 mV. The lower traces are averages (12) of the currents recorded simultaneously from each recording electrode ∼7 min after establishing whole-cell recording in both cells. The magnitude of the outward current drawn from one cell by hyperpolarizing the other indicates a coupling conductance of ∼300 pS which was present for the duration of the recording (∼12 min). The ACSF contained 0.5 μm TTX.