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. 1998 Jan 1;506(Pt 1):3–11. doi: 10.1111/j.1469-7793.1998.003bx.x

Figure 2. Prepulse potentiation of α1B modulated by co-expressed Gβγ.

Figure 2

The VDCC α1 subunit α1B was co-expressed with the VDCC accessory subunits α2-δ and β2a and the G protein subunits Gβ1 and Gγ2 in COS-7 cells, as described previously (Page et al. 1997). The cell was held at -100 mV, and the voltage was stepped according to the protocol shown, with two 50 ms steps P1 and P2 to varying voltages (-40, -30 and -20 mV) separated by an 80 ms step (prepulse) to a large depolarizing voltage (+120 mV). Leak subtraction was performed according to a P/8 protocol. The amplitude of the currents in P2 after the depolarizing prepulse are clearly larger than in P1 before the prepulse and their rate of activation is faster at all potentials (from data supplied by Dr G. J. Stephens).