Effects of mucosal amiloride and TEA (added subsequently) after 6 h incubation with aldosterone (3 nM), on ISC and tissue conductivities (G) (n = 10). Blocking of TEA-sensitive ISC was accompanied by a drop in Gs, indicating that the aldosterone-induced conductivity was restricted to surface epithelium. n.s., not significantly different; *P < 0.05; **P < 0.01; ***P < 0.001 (compared with the preceding condition); †, value subjected to Bonferroni correction, if significant.