Figure 13. Real time Ca²⁺ influx into mitochondria.

Depolarization to +110 mV was applied to a ferret cell with Mn²⁺ quench of cytosolic indo-1. [Ca²⁺]_m and [Ca²⁺]_CL are estimated by ΔL and mitochondrial indo-1 fluorescence using eqns (7) and (18), respectively. The net mitochondrial Ca²⁺ influx, Q_Ca,m, was measured by the Ca²⁺-sensitive indo-1 signal, ΔF_d (see eqn (17)). Scale for Q_Ca,m is given in both Ca²⁺ charge (30 pC) and total mitochondrial Ca²⁺ concentration (17 μm), assuming a total mitochondrial volume of 9 pl for this cell.