Figure 1. Schematic representation of the transverse brainstem slice obtained from mice together with an intracellular recording from a rhythmically active neurone.

Transverse slice, encircled area indicates the location of the pre-Bötzinger complex (PBC). The arrow above the schematic electrode points toward a whole-cell patch recording from an inspiratory neurone (IN) recorded in the pre-Bötzinger complex of a P15 mouse (upper trace) and simultaneously with hypoglossal (XII) rootlet activity using a suction electrode (lower trace). Abbreviations: IO, inferior olive; NA, nucleus ambiguus; NTS, nucleus of the tractus solitarius; Sp5, spinal trigeminal nucleus; XII, hypoglossal motor nucleus; XII nerve, hypoglossal rootlet.