Figure 2.
Only certain AMP analogues block activation of transducin by DEN plus taste membranes. Taste membrane-dependent activation of transducin by DEN (5.0 mM) is not inhibited by adenosine 5′-carboxylate (ACA, 5.0 mM), adenosine 5′-monosulfate (AMS, 5.0 mM), theophylline (THE, 5.0 mM), adenine hydrochloride (ADE, 5.0 mM), adenosine hydrochloride (ADO, 5.0 mM), cAMP (5.0 mM), or caffeine (CAF, 5.0 mM). DEN/taste membrane activation of transducin is inhibited by thymidine 5′-monophosphate (TMP, 5.0 mM), 5′-cytidylic acid (CMP, 5.0 mM), inosinic acid (IMP, 5.0 mM), ADP (5.0 mM), 3′AMP (5.0 mM), adenosine 5′-succinate (ASU, 5.0 mM) and ATP (5.0 mM). H2O and rhodopsin (RHO) lanes control for nonspecific receptor-independent effects.