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Colonic LMV and homogenate (25 μg of protein each) were separated by 8% SDS-PAGE and electrotransferred to a nitrocellulose membrane. Immunoblotting was carried out as described in Methods. A, immunodetection of NHE2; B, immunodetection of NHE3. Lane a, pig colonic homogenate; lane b, pig colonic LMV; lane c, human colonic homogenate; lane d, human colonic LMV.
