Figure 2. Effect of glycine on LSO neurones while synaptic transmission and spike activity were blocked.

Glycine (1 mm) was applied to the bath as indicated by the bars. A, responses obtained in the perforated-patch mode (GPP); B, responses recorded in the conventional whole-cell configuration (WCC). The current responses were almost unchanged in a nominally Ca²⁺-free saline which contained 10 mm Mg²⁺ and 300 nm tetrodotoxin to block synaptic transmission and neuronal spike activity, indicating that glycine acted directly on the neurone under investigation.