Figure 3. Temperature dependence of the steady-state current.

Current-voltage relationship of the steady-state current measured at 20 ms of varying voltage steps for wild-type (^) and Ile693Thr mutant (•) channels. A, data obtained at 20–22 °C for wild-type (n = 6) and mutant channels (n = 6). B, data obtained at 13–15 °C for wild-type (n = 5) and mutant channels (n = 6). C, steady-state currents measured at 200 ms following voltage steps to −10 mV. Recordings were performed at room temperature or after cooling to 13–15 °C as indicated. At both command potentials, open columns represent data obtained for wild-type (n = 6 and n = 6, at 20–22 °C and 13–15 °C, respectively), and closed columns show data obtained for Ile693Thr mutant (n = 4 and n = 6 at 20–22 °C and 13–15 °C, respectively).