Figure 3. Upregulation of hPepT1 mRNA by Gly-Gln.

Northern blots of poly(A)⁺ RNA, prepared from Caco-2 cells grown either under control conditions or in medium supplemented with 4 mm Gly-Gln, probed separately with ³²P-labelled hPepT1, sucrase-isomaltase (SI) and β-actin cDNAs. Poly(A)⁺ RNA was prepared 0, 15 and 24 h after the addition of 5 μg ml⁻¹ actinomycin D. Each lane was loaded with 5 μg poly(A)⁺ RNA. Blots were washed at 47 °C in 2 × SSC.