Figure 4.

Phosphorylation-mimic S3D cofilin potentiates PLD1 stimulation by carbachol and rescues PLD1 stimulation in cofilin-depleted cells. HEK-293 cells or N1E-115 cells were transfected with phosphorylation-mimic S3D cofilin, or with empty vector (Control) (A), or transfected with either phosphorylation-mimic S3D mouse cofilin, human cofilin siRNA pSUPER plasmid (siRNA hCofilin), or both constructs together (B). After 48 h, stimulated [³H]PtdEtOH accumulation was determined in the presence of 1 mM carbachol (Carb). Data shown are means±s.e. (n=3) (A), or are representative of three experiments (B). ^*P<0.05.