Figure 5. Effects of serotonin on AMPAR- and NMDAR-mediated currents activated by fast application of glutamate to outside-out patches.

A, schematic drawings of the methodological principle of the fast application system (representative camera lucida drawing of a layer III projecton neuron). B, 5-HT (50 μm) had no effect on isolated AMPAR-mediated currents when elicited by a fast application of glutamate (10 mm) in the presence of 60 μm APV in both barrels. C, glutamate-activated currents in the presence of 20 μm NBQX and 10 μm glycine, while the external Mg²⁺ was omitted (both barrels). Again, 5-HT did not show any effects on the NMDAR-mediated currents.