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. 1998 Apr 15;508(Pt 2):379–392. doi: 10.1111/j.1469-7793.1998.379bq.x

Figure 1. Wild-type murine CFTR forms a channel regulated by cAMP-dependent phosphorylation and intracellular ATP.

Figure 1

Representative records are from an excised inside-out membrane patch from a CHO cell stably expressing wild-type murine CFTR. ATP (0.3 mM or 1 mM) and the catalytic subunit of PKA (75 nM) were present in the intracellular solution during the times indicated. Each trace is 5 s long. Voltage was clamped at -50 mV, and there was a large Cl− concentration gradient across the membrane patch (external [Cl−], 10 mM; internal [Cl−], 147 mM). Dashed lines indicate the closed channel state and downwards deflections correspond to channel openings. For the purpose of illustration, records were filtered at 500 Hz and digitized at 1 kHz.