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. 1998 May 1;508(Pt 3):667–680. doi: 10.1111/j.1469-7793.1998.667bp.x

Figure 5. MHC mRNA transcripts and protein isoforms in single fibres obtained from the slow region of the anterior tibialis muscle.

Figure 5

Expression of MHC mRNA transcripts and protein isoforms in immunohistochemically identified frog fibre types obtained from the slow region of the anterior tibialis muscle (labelling scheme as in Fig. 4). A and B, serial sections of anterior tibialis muscle stained for reactivity to the MHC monoclonal antibodies D5 (A) and MHC-S (B). Scale bar, 100 μm. Immuno-classified tonic fibres (no. 15 and no. 17) expressed only the RPMT mRNA (C) and contained a single MHC protein band migrating at the position of the middle band of the triplet seen in whole AT muscle. The type 3 fibre (no. 16) coexpressed RPM3 and RPM2 transcripts and contained 2 protein bands migrating at the position of the upper and lower bands. The type 2 fibre (no. 14) expressed RPM2 and a single protein isoform at the position of the upper band.