Table 1.

Histochemical and immunohistochemical reactivity of frog muscle fibres

Treatment	Type 1	Type 2	Type 3	Tonic
mATPase at pH 4.8	+	0	0	+
mATPase at pH 10.1	0	+	0	0
F8 antibody	+	0	+	0
MHC-S antibody	0	0	0	+
NA8 antibody	0	+	+	+
D5 antibody	0	0	+	0

The table shows differential reactivity of frog fibre types for mATPase and MHC monoclonal antibodies in the AT muscle of R. pipiens. The staining for each reaction is scored as either positive (+) or negative (0). Dilutions for the antibodies were as follows: F8, 1:30 000; NA8, 1:20000; MHC-S, 1:500; and D5, 1:15, supernatant. The antibodies were all anti-rat except NA8, which was anti-chicken. F8 and D5 were a gift of Professor S. Schiaffino (University of Padua, Italy) and NA8 was a gift of Dr E. Bandman (University of California, Davis, CA, USA). MHC-S was obtained commercially (Vector Laboratories).