Table 2.

Clone-specific primer pairs and corresponding PCR product length

PCR primer	Nucleotide sequence	5′ start position	PCR product length (bp)
RPM1 (S)	5′-gaatctgaggaacaagccaatgc-3′	346	133
RPM1 (AS)	5′-tgatatcccggctcttgactctc-3′	478
RPM2 (S)	5′-agtggaggaacaggctac-3′	348	251
RPM2 (AS)	5′-tatttgcacctagagctacac-3′	598
RPM3 (S)	5′-ttgaggagcaatccaatgtta-3′	397	178
RPM3 (AS)	5′-caccttgaggctcagtcact-3′	574
RPMT (S)	5′-acgaatgcaggacttgatag-3′	329	290
RPMT (AS)	5′-agggattgtgaagtctgtctc-3′	618

The PCR primer pairs (sense (S) and antisense (AS)) used for clone-specific amplification of four MHC transcripts. The 5′ position of each primer (as numbered in Fig. 1) is indicated along with the expected PCR product length for each primer pair.