Table 1.
Composition of solutions (mM)
| External solutions | NaCl | KCl | CaCl2 | MgCl2 | Hepes | Pipes | Glucose |
|---|---|---|---|---|---|---|---|
| 5K standard | 160 | 5 | 2.5 | 1 | 5 | — | 10 |
| 5K Mg-free | 160 | 5 | 2.5 | — | 5 | — | 10 |
| HGS | 130 | 4.5 | 2 | — | — | 5 | 30 |
| Internal solutions | KCl | KGlu | CsCl | NaCl | MgCl2 | KOH | CsOH | EGTA | Hepes |
|---|---|---|---|---|---|---|---|---|---|
| 160 KCl | 160 | — | — | — | — | 15 | — | 5 | 10 |
| 160 CsCl | — | — | 160 | — | — | — | 15 | 5 | 10 |
| 132 KGlu | 20 | 132 | — | 6 | 1 | 15 | — | 1 | 10 |
| 155 KGlu | — | 155 | 5 | — | — | — | 15 | 5 | 10 |
| 140 KGlu | — | 140 | 20 | — | — | — | 15 | 5 | 10 |
pH was adjusted to 7.4 by NaOH and Hepes for 5K standard and 5K Mg-free; by NaOH and Pipes for HGS (high-glucose saline); by KOH and Hepes for 160 KCl and 132 KGlu; and by CsOH and Hepes for 160 CsCl, 155 KGlu and 140 KGlu. KGlu, potassium gluconate.