Table 1.
TNF per well | TNF-bp per well | Detectable TNF per well |
---|---|---|
20 ng | 500 ng | n.d. |
20 ng | 250 ng | n.d. |
20 ng | 125 ng | n.d. |
20 ng | 62.5 ng | n.d. |
20 ng | 50 ng | n.d. |
20 ng | 25 ng | n.d. |
60 pg | — | 65 pg |
20 pg | — | 16 pg |
Amounts of bioactive TNF recovered after incubation for 24 h with different concentrations of TNF-bp. Each sample was analysed in triplicate. n.d., not detectable.