Figure 6. Intracellular pH changes in cultured Xenopus myocytes.
Cell cultures were loaded with 5 μM BCECF AM for 5-10 min at room temperature (20-22 °C) in Ringer solution and then washed with plain Ringer solution 3 times. Bath application of NH4Cl resulted in concentration-dependent intracellular alkalinization of 1-day-old Xenopus cultured myocytes (A and C). Substitution of all extracellular NaCl with sodium acetate caused intracellular acidification (B and C). Changes of the extracellular pH between pH 6.6 and 8.6 had no significant effect on intracellular pH (A-C). Data are presented as means ± s.e.m (n). *P < 0.05 compared with pH 7.6 normal Ringer solution.