▪, glomeruli; , proximal tubules; □, TIS samples. t-SOD, manganese + copper/zinc superoxide dismutase; MnSOD, manganese superoxide dismutase. *P < 0.01vs. glomeruli; **P < 0.01vs. glomeruli and vs. proximal tubules.
Analysis of superoxide dismutases and catalase by Western blotting revealed higher enzyme protein levels in PT than in glomeruli (Fig. 2). Similarly, steady-state mRNA levels of these enzymes were significantly higher in PT than in glomeruli (Fig. 3). Among the different glutathione peroxidases, mRNA analysis was confined to cellular GSH-Px I. In contrast to results for SOD and catalase, GSH-Px mRNA levels in proximal tubules and glomeruli were the same.