Figure 4. Slow recovery phase in CO₂/HCO₃⁻ buffer is also Cl⁻_o dependent.

Two types of alkali load are investigated, simulated metabolic (A) and respiratory (B) alkalosis. A, acetate pre-pulsing to raise pH_i. Note slow recovery phase in Cl⁻-free solution on Cl⁻_o re-admission; in this case only 90.5 mM Cl⁻_o re-admitted (remaining anion balance being gluconate) B, P_CO2 was elevated to 10 % (extracellular bicarbonate raised to 44 mM; constant pH_o of 7.40) for several minutes and then reduced back to 5 % (22 mM HCO₃⁻) to induce intracellular alkalosis. Recovery only occurred upon readmission of Cl⁻_o.