Figure 7. α5 deletion removes all of the native 50 pS conductance channels.

Single channel recording from control and α5 antisense oligonucleotide-treated neurones (cell attached configuration; 2.5 μM ACh). A, left panel, all point histograms of ACh-gated channels in patches from control neurones (V_h = +60 mV). Right panel, determination of the slope conductances of the current amplitude detected from the all point histogram at distinct holding potentials indicates three dominant classes of channels (15, 35 and 50 pS) with less frequent events of ≈18 pS channels. B, all point histogram (left) and slope conductance determination (right) of ACh-gated channels in patches from α5 minus neurones. The patches are dominated by 18, 35 and 65 pS channels with less frequent openings of the 15 pS class. The 50 pS conductance was never detected in α5 minus neurones.