Figure 5. Influences of FK-506 on HFS effects in differentiated NG108-15 cells.

I-V recordings of pharmacologically separated L-type VOCCs by 1 μM ωCgTX GVIA (A) and N-type VOCCs by 10 μM nifedipine (B) were obtained in control conditions (curves 1 and 3) and immediately after HFS (curves 2 and 4) in cells which were patched with a pipette solution containing 2 μM FK-506. Amplitude histograms (means ±s.d.) for integral of the I-V relationships (Σ_Ca) in FK-506-containing solution (C and D) and similar histograms obtained in control conditions (E and F) are presented. Bath solutions contained 0.1 mM Ni²⁺.