A-D, ACh (10−6 M) was applied for 1 min in the absence (A; Control) and presence (31 min, B; 80 min, C) of 10−5 M clotrimazole, and 65 min after removal of clotrimazole from the superfusate (D). E-G, ACh was applied for 1 min before (E; Control) and after application of SKF 525a (3 × 10−5 M for 71 min, F; 10−4 M for 25 min, G). Traces shown in A-D and E-G were recorded from single cells in different vessels.