ACh (10−6 M) was applied for 1 min before (A; Control), during (8 min, B; 30 min, C) application of 5 × 10−8 M CTX (applied at the point indicated in B), and after removal of CTX for 70 min (D), or before (E; Control) and during (F) application of 10−7 M apamin for 7 min. Traces in A-D and E and F were recorded from single cells in different tissues.