Table 1.
Comparison of the percentage of responding (increase in [Ca2+]i) confluent or dissociated astrocytes stimulated by superfusion or focal application of receptor agonists
Cells responding to receptor agonist | |||||
---|---|---|---|---|---|
Method of application | Cells tested | Treatment | 1 mM carbachol (%) | 0.1 mM methoxamine (%) | 0.1 μM Et1 (%) |
Superfusion | Confluent | Control | 60 (743) | 70 (364) | 93 (83) |
Confluent | 10 μM αGA | 38 (469) | 41 (222) | 90 (69) | |
Dissociated | Control | n.d. | 32 (41) | n.d. | |
Focal | Confluent | Control | 43 (42) | 51 (35) | 98 (11) |
Numbers in parentheses indicate the number of cells which have been investigated in each condition. n.d., not determined