Figure 2. Recovery from fast inactivation was accelerated for both V1293I and S804F.

A two-pulse voltage protocol using a 30 ms conditioning pulse to -10 mV followed by a variable recovery interval at -90 mV was used to measure recovery from fast inactivation. V1293I currents recovered 3-fold faster than WT. S804F channels recovered 1.4-fold faster than WT. Current traces have been normalized in amplitude. Peak currents were -12.8 nA for WT, -9.4 nA for S804F and -13.3 nA for V1293I.