Figure 1. Effect of full metabolic inhibition on L-type Ca²⁺ current.

A, once the whole-cell configuration was achieved, I_Ca was sampled at 0.2 Hz and I-V relationships were recorded at the times indicated by the open and filled circles. The typical raw traces (inset) indicate that I_Ca run-down was minor over the 10 min sampling period. B, mean I-V relationships showing that sampling I_Ca at 0.2 Hz for 10 min did not significantly reduce I_Ca under our conditions (n= 4). C, I_Ca was sampled for 5 min before the indicated interruption in order to record a control I-V relationship. This was followed by external wash-in (arrow) of 2-deoxyglucose (10 mM) and sodium cyanide (2 mM) (full metabolic inhibition, FMI). The raw traces (inset) demonstrate that I_Ca prior to FMI (open circle) was larger than I_Ca after FMI (filled circle). D, mean I-V relationships which show that, on average, 10 min of FMI resulted in a 40 % reduction of I_Ca at +10 mV (n= 5, P < 0.01).