Table 1.
Effect of thyrotropin-releasing hormone on HERG kinetic parameters
| Treatment | External solution | Activation t½ (ms) | n | N | Deactivation τ (ms) | Inactivation recovery τ (ms) | n | N |
|---|---|---|---|---|---|---|---|---|
| Control | 50 mm K+ | 30.6 ± 3.5 | 21 | 3 | 589 ± 64 | 9.4 ± 0.4 | 48 | 7 |
| TRH | 50 mm K+ | 66.0 ± 10.0* | 21 | 3 | 348 ± 30† | 10.4 ± 0.5 | 48 | 7 |
| Control | Cs+ | 13.3 ± 0.8 | 3 | — | 100 ± 4 | 14.2 ± 2.6 | 3 | — |
| TRH | Cs+ | 19.5 ± 1.8* | 3 | — | 64 ± 3* | 12.6 ± 2.7 | 3 | — |
*
P < 0.05
†
P < 0.001 vs. control
Student's paired t test. Times for half-maximal current activation (t½) were obtained from envelope of tail currents following the voltage protocol shown at the top of Fig. 2. Deactivation and inactivation recovery time constants (τ) were measured from bi-exponential fits to tail currents after repolarizing the membrane to −100 mV. n represents the number of oocytes used for the number of donors indicated by N.