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Caffeine (30 mM), InsP3 (100-300 μm), cADPR (10-300 μm), and CCh (1 μm) were added to the α-toxin-permeabilized rabbit tracheal smooth muscle (SM, A), human bronchial SM (B), and guinea-pig ileum longitudinal SM (C). Note that GTP was present with InsP3 and cADPR in ileum SM. The traces represent at least three separate experiments.
