Table 2.

Effects of cADPR- and NAADP-related compounds on CCh-induced contraction via Ca²⁺ release in tracheal SM permeabilized with α-toxin

Reagent	Dose	30 mm caffeine response (% of maximum contraction)	1 μm CCh (% of caffeine response)	n
8-NH2-cADPR	100 μm	54.1 ± 5.9	98.2 ± 6.3	5
	Control	53.3 ± 6.6	111 ± 12	6
Ruthenium Red	100 μm	56.4 ± 5.4	125 ± 8.9	4
	Control	60.0 ± 4.2	118 ± 14.5	4
FK506	30 μm	59.3 ± 8.5	95.9 ± 9.1	4
	Control	55.2 ± 9.0	92.3 ± 8.0	4
α-NAD+	2.5 mm	61.2 ± 8.0	111 ± 18	4
β-NAD+	2.5 mm	59.3 ± 6.8	105 ± 14	6
	Control	58.0 ± 6.5	109 ± 19	4
Procaine	20 mm	23.0 ± 3.2 *	0.5 ± 0.7 *	4
	60 mm	0.2 ± 0.3 *	n.d.	4
	Control	59.3 ± 8.5	105 ± 10	4
NAADP	100 μm	60.2 ± 7.6	115 ± 30	4
thio-NADP	100 μm	59.8 ± 7.6	138 ± 14	4
	Control	63.2 ± 8.5	127 ± 5.5	4

Data are presented as means ±s.e.m. and were compared by Mann-Whitney U test or Student's t test with the Bonferroni correction as appropriate. A P value of < 0.05

^*was considered statistically significant. CCh, carbachol. n.d., not determined.