Table 1.

Recovery of the response to caffeine after 30 s washout of P_i under various myoplasmic conditions

Washout conditions	Mean response to caffeine (% of controls)
ATP	60 ± 5 (14)
No ATP	73 ± 4 (7)
No ATP + 30 mm Pi	32 ± 5 (4)****
No ATP + 2 mmPHPA	65±3(3)
ATP + 100 μm DIDS	79 ± 4 (4)***
ATP + 100 μm 9-AC	74 ± 7 (3)

The mean response to caffeine indicates the peak force response to caffeine after washout of P_i for 30 s (as detailed in Fig. 3) as a percentage of the control responses before and after P_i exposure. Washout conditions: ATP refers to a standard K-HDTA solution; No ATP refers to a rigor solution with or without various modifications. All solutions contained 125 μm total EGTA. Values represent the mean response ±s.e.m.with the sample size noted in parentheses. Asterisks represent a significant difference from the response to caffeine following washout under normal myoplasmic conditions (ATP)

^***P < 0.01

^****P < 0.001