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. 1999 Aug 17;96(17):9926–9931. doi: 10.1073/pnas.96.17.9926

Figure 1.

Figure 1

(A) Amino acids in the amino- and carboxyl-terminal domains of IRK1J subunit that were individually mutated to cysteine. The amino- and carboxyl-terminal sequence is from residues 54–86 and 213–234, respectively. Mutation of the underlined residues to cysteine resulted in nonfunctional homomeric channels. (B–E) Effect of internal MTSET on E224C or V227C channels. (B and D) Time course of inhibition of inward current (sign reversed) at −80 mV by 2 mM MTSET, applied for 1 (B) or 2 (D) min to the intracellular side of a giant inside-out patch. Current sampling interval was changed to 1 s during MTSET application in B. (C and E) Current–voltage (I–V) relations from the corresponding patch, before (trace a), during (trace b), and after (trace c) MTSET application. Current was generated by voltage ramps from −100 mV to +80 mV over 70 ms. Notice that the I–V relation for V227C channels obtained in the presence of MTSET exhibits strong inward rectification (trace b), which is reversible after washout of MTSET (trace c).