Figure 4.

Differential effect of MTESA and MTSET. (A) Time course of irreversible inhibition of F58C channels by 2 mM internal MTSET. (B) Effect of internal MTSEA and then MTSET on F58C channels from another patch. Notice that after a 2-min application of 2.5 mM MTSEA, subsequent application of 2 mM MTSET no longer produced irreversible inhibition of the current. In both cases, current was elicited by voltage ramps from −100 mV to +80 mV over 70 ms from a holding potential of −40 mV at a 4-s interval, and the current amplitude at −80 mV was plotted.