Figure 6. Effect of chronic hypoxia on adenylate cyclase activity in PC12 cells.

Cells were harvested at 24 and 48 h after exposure to 10 % O₂ and the production of cAMP stimulated by forskolin (2 μm) or adenosine (50 μm) was measured. Basal production of cAMP was unchanged in response to 10 % O₂ for either 24 or 48 h (left). Hypoxic exposure did not alter the activity of adenylate cyclase stimulated by forskolin (right). In addition, there was no significant change in the production of cAMP stimulated by adenosine in response to chronic hypoxia (middle). Each bar shows the mean + s.e.m. of 6 samples from 2 separate experiments.