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The shifts of intracellular pH (pHi) induced by external pH changes before (A) and after (B) reducing the residual HCO3− concentration by gassing the Hepes-buffered saline with 100 % O2 (see also Methods) are shown. The recording in C shows the pHi shifts again in an ungassed, nominally CO2/HCO3−-free saline in the same experiment.
