Figure 5. Typical OG-5N transient evoked by Ca²⁺ influx through L-type channels.

A and B, OG-5N transients (A) and I_Ca (B) at 0 mV before (Control) and after 10 mm caffeine. The OG-5N signal in the presence of caffeine was averaged from 5 consecutive recordings. C, OG-5N transient (noisy trace) obtained in the presence of caffeine was scaled and superimposed on the inverted I_Ca (A.U., arbitrary units). D, same as in C except that the integral component of the OG-5N transient was subtracted (α = 6.0 s⁻¹). See the text for the algorithm.