Figure 9. Immunocytochemistry and immunoprecipitation analysis for CFTR protein expression.

A, C and E, light micrographs of a villus fragment (A), a fragment consisting of a lower villus and upper or whole crypts (C) and a whole-crypt fragment (E) isolated from guinea-pig small intestine. Scale bars, 50 μm. B, D and F, indirect immunofluorescence micrographs of epithelial fragments corresponding to A, C and E, respectively. Immunocytochemistry was performed with anti-CFTR antibody. G, immunoprecipitation of CFTR. Isolated epithelial fragments were solubilized using detergent and CFTR was immunoprecipitated with anti-CFTR antibody. Data represent duplicate experiments.