Figure 1. Response of an outside-out patch to application of 10 μm FLRFamide.

FLRFamide evoked inward single-channel currents throughout the periods of application. Arrows above the current trace show when the peptide was applied. The patch contained several peptide-gated channels. The difference in the current traces before (a) and during (b) application are shown on an expanded time scale. Channel openings are downwards. The baseline is shown by the dashed line. The membrane potential was −80 mV and the recording was low-pass filtered at 1 kHz (−3 dB).