Table 1.
n | Perfusion rate (nl min−1) | (C/P)In | Jv (nl min−1) | Collectate [Na7plus;] (mmol l−1) | JNa (pmol min−1) | Collectate [K+] (mmol l−1) | JK (pmol min−1) | |
---|---|---|---|---|---|---|---|---|
Standard perfusate | ||||||||
Control | 34 | 19.7 ± 0.3 | 1.69 ± 0.06 | 7.7 ± 0.4 | 69 ± 3 | 1971 ± 71 | 2.5 ± 0.2 | 47 ± 2 |
EIPA | 28 | 19.6 ± 0.3 | 1.61 ± 0.05 | 7.4 ± 0.4 | 76 ± 4 | 1851 ± 90 | 2.6 ± 0.1 | 44 ± 2 |
Low-Na+ perfusate- | ||||||||
Control | 46 | 19.9 ± 0.2 | 1.08 ± 0.01 | 1.5 ± 0.1 | 73 ± 2 | 828 ± 41 | 2.4 ± 0.1 | 28 ± 2 |
EIPA | 40 | 20.2 ± 0.2 | 1.08 ± 0.01 | 1.4 ± 0.2 | 80 ± 2 * | 726 ± 37 * | 2.5 ± 0.1 | 27 ± 2 |
Low-Na+ perfusate + bumetanide- | ||||||||
Control | 32 | 19.8 ± 0.2 | 1.02 ± 0.01 | 0.4 ± 0.2 | 108 ± 1 | 86 ± 27 | 4.7 ± 0.2 | −20 ± 3 |
EIPA | 30 | 20.3 ± 0.4 | 1.00 ± 0.01 | 0.1 ± 0.2 | 112 ± 2 | −24 ± 18 * | 4.5 ± 0.1 | −18 ± 2 |
Low-Na+, high-bicarbonate perfusate- | ||||||||
Control | 15 | 19.9 ± 0.3 | 1.04 ± 0.02 | 0.8 ± 0.2 | 68 ± 2 | 887 ± 42 | 2.6 ± 0.2 | 24 ± 3 |
EIPA | 16 | 19.8 ± 0.3 | 1.06 ± 0.01 | 1.1 ± 0.2 | 80 ± 1† | 689 ± 24† | 2.6 ± 0.1 | 25 ± 3 |
Values are means ±s.e.m.n, number of tubules perfused; (C/P)In, collectate/perfusate [14C] inulin concentration ratio; Jv, net water flux; JNa, net sodium flux;JK, net potassium flux; EIPA, ethyl isopropyl amiloride.
P < 0.05
P < 0.001 compared with corresponding value in the absence of EIPA.