Figure 2. Effect of 25 μmmu;mol l⁻¹ ryanodine on Bk-induced changes of [Ca²⁺]_pn in single freshly isolated bovine left circumflex endothelial cells in 145 (panel A) and 19 (panel B) mmol l⁻¹ Na⁺-containing solution.

Freshly isolated endothelial cells were loaded with fura-2 to measure [Ca²⁺]_pn. Panel A, in Ca²⁺-free HBS containing 145 mmol l⁻¹ Na⁺ without (control, ○, continuous line) or with 25 μmmu;mol l⁻¹ ryanodine (•, dashed line) endothelial cells were stimulated with 100 nmol l⁻¹ Bk followed by the addition of 2.5 mmol l⁻¹ Ca²⁺ as indicated. Panel B, in Ca²⁺-free HBS containing 19 mmol l⁻¹ Na⁺ without (control, ○, continuous line) or with 25 μmmu;mol l⁻¹ ryanodine (•, dashed line) cells were stimulated with 100 nmol l⁻¹ Bk followed by the addition of 2.5 mmol l⁻¹ Ca²⁺. As indicated, [Na⁺]_o was increased to 145 mmol l⁻¹. Points represent means ±s.e.m. (panel A, n= 36; panel B, n= 32).