Table 2.

Peak change (Δ) cytosolic [Ca²⁺] of TM3 cells under different experimental conditions

Protocol	Concentration	Δ[Ca2+]1 (nm)
Ni2+ (control)	5 mm	1371 ± 393
Caffeine	250 μm	66.8 ± 13.61
	500 μm	46.4 ± 10.52
Ni2+ after caffeine	250 μm	136 ± 44.03
	500 μm	401 ± 2164
db-cAMP	200 μm	0
Ni2+ after db-cAMP	200 μm	2443 ± 6855

Statistics by ANOVA with Bonferroni's correction for inequality. Basal compared with peak cytosolic [Ca²⁺]:

¹P = 0.004

²P = 0.010. Peak δ[Ca²⁺]₁ of treatment compared with Ni²⁺ alone (control):

³P = 0.006

⁴P = 0.057

⁵P = 0.274 (n = 3–6 cells for each variable). db-cAMP, dibutyryl cyclic AMP.